NEET · BiologyHard
Which of the following statements correctly describes the role of the Rho protein in bacterial transcription termination?
- A.It binds to the DNA template to stall RNA polymerase
- B.It acts as an ATP-dependent helicase to separate the RNA-DNA hybrid
- C.It forms a hairpin loop structure in the nascent RNA
- D.It competes with sigma factor for binding to the promoter
Show correct answer & step-by-step solution
Correct answer: B — It acts as an ATP-dependent helicase to separate the RNA-DNA hybrid
Solution
- Rho-dependent termination requires the Rho protein to bind to a specific sequence on the nascent RNA.
- Rho moves along the RNA transcript toward the RNA polymerase.
- Upon reaching the transcription bubble, Rho uses its ATPase activity to unwind the RNA-DNA hybrid.
- This releases the RNA transcript from the DNA template.
- Hence the answer is (B).
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