NEET · BiologyMedium
Which technique is primarily used to separate DNA fragments based on their size and charge?
- A.
- B.
- C.
- D.
Show correct answer & step-by-step solution
Correct answer: B —
Solution
- DNA molecules are negatively charged due to the phosphate backbone.
- When placed in an electric field, DNA moves toward the positive electrode (anode).
- The agarose gel matrix acts as a molecular sieve.
- Smaller fragments move faster through the pores than larger fragments, allowing separation by size.
- Hence the answer is (B).
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