NEET · BiologyHard
In the production of recombinant human insulin, the A and B polypeptide chains are produced separately in E. coli. What is the final step required to form the functional hormone?
- A.Proteolytic cleavage of the C-peptide from the proinsulin molecule
- B.Formation of disulfide bonds between the A and B chains in vitro
- C.Glycosylation of the polypeptide chains in the bacterial cytoplasm
- D.Removal of the signal peptide sequence by bacterial signal peptidases
Show correct answer & step-by-step solution
Correct answer: B — Formation of disulfide bonds between the A and B chains in vitro
Solution
- Human insulin is synthesized as a precursor called proinsulin containing A, B, and C chains.
- Bacteria lack the machinery to excise the C-peptide from proinsulin.
- Therefore, the A and B chains are expressed as separate fusion proteins in E. coli.
- These chains are purified and linked together by creating disulfide bonds in a controlled chemical environment.
- Hence the answer is (B).
Attempt this question & track your score
Sign up free to answer, get instant scoring, and let SolveGini track which Biology topics you need to revise.
Attempt & Track Free →More Biotechnology practice questions
- What is the primary function of DNA ligase in recombinant DNA technology?
- Which technique is used to separate DNA fragments based on their size?
- Which of the following is commonly used as a cloning vector in biotechnology?
- What is the role of the Taq polymerase enzyme in the polymerase chain reaction?
- Which enzyme is commonly known as molecular scissors in biotechnology?
- In the process of Agrobacterium-mediated transformation, which part of the Ti plasmid is transferred into the host plant
- A researcher wants to amplify a specific DNA segment using PCR. If the initial number of DNA molecules is 10, how many m
- Which of the following is a selectable marker used in the pBR322 plasmid vector?
- Which technique is primarily used to separate DNA fragments based on their size and charge?
- In the context of recombinant DNA technology, which of the following enzymes is used to create blunt ends in DNA fragmen
- In the process of RNA interference (RNAi) used for silencing genes in nematodes, what is the role of the enzyme Dicer?
- Consider a cloning vector with two antibiotic resistance genes, Ampicillin (AmpR) and Tetracycline (TetR). If a foreign