NEET · BiologyHard
Which of the following statements accurately describes the function of the Taq polymerase enzyme during the extension phase of PCR?
- A.It adds deoxyribonucleotides to the 3' hydroxyl end of the primer using the template strand
- B.It synthesizes the primer required for the initiation of DNA replication
- C.It proofreads the newly synthesized DNA strand to ensure high fidelity
- D.It unwinds the double-stranded DNA template at high temperatures
Show correct answer & step-by-step solution
Correct answer: A — It adds deoxyribonucleotides to the 3' hydroxyl end of the primer using the template strand
Solution
- PCR requires a thermostable DNA polymerase to withstand high temperatures.
- During the extension phase, the enzyme binds to the primer-template complex.
- It catalyzes the addition of dNTPs to the 3' OH end of the primer.
- This synthesis proceeds in the 5' to 3' direction along the template strand.
- Hence the answer is (A).
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