NEET · BiologyEasy
What is the primary function of DNA ligase in recombinant DNA technology?
- A.To cut DNA at specific sites
- B.To amplify DNA segments
- C.To join DNA fragments together
- D.To separate DNA fragments by size
Show correct answer & step-by-step solution
Correct answer: C — To join DNA fragments together
Solution
- DNA ligase is an enzyme that repairs breaks in the DNA backbone.
- It catalyzes the formation of a phosphodiester bond between the -hydroxyl and -phosphate ends of DNA.
- This process is essential for sealing nicks in recombinant DNA molecules.
- Hence the answer is (C).
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