NEET · BiologyHard
Consider a cloning vector with two antibiotic resistance genes, Ampicillin (AmpR) and Tetracycline (TetR). If a foreign DNA fragment is inserted into the BamHI site located within the TetR gene, what will be the phenotype of the recombinant colonies?
- A.They will grow on both Ampicillin and Tetracycline media
- B.They will grow on neither Ampicillin nor Tetracycline media
- C.They will grow on Ampicillin but not on Tetracycline media
- D.They will grow on Tetracycline but not on Ampicillin media
Show correct answer & step-by-step solution
Correct answer: C — They will grow on Ampicillin but not on Tetracycline media
Solution
- The vector contains two resistance markers, AmpR and TetR.
- Insertion of DNA into the BamHI site within the TetR gene disrupts the coding sequence.
- This process, known as insertional inactivation, results in the loss of tetracycline resistance.
- The AmpR gene remains intact, allowing growth on ampicillin-containing media.
- Hence the answer is (C).
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